• Characteristics of the Microbial Community Responding to the Vertical Distribution of TPH Concentrations in the Petroleum-Contaminated Site

  • Soo Min Song1,2·Hee Sun Moon1,2*·Ji Yeon Han3·Jehyun Shin1·Seung Ho Jeong1·Chan-Duck Jeong4·Sunghyen Cho5

  • 1Groundwater Environment Research Center, Climate Change Response Division, Korea Institute of Geoscience and Mineral Resources (KIGAM)
    2Geological Science, University of Science and Technology (UST)
    3Hanwool Life Science Ltd.
    4Future Rural Research Office, Rural Research Institute, Korea Rural Community Corporation
    5National Instrumentation Center for Environmental Management (NICEM), Seoul National University

  • 유류오염지역 부지 내 TPH 수직 농도 분포에 따른 미생물 생태 특성

  • 송수민1,2·문희선1,2*·한지연3·신제현1·정승호1·정찬덕4·조성현5

  • 1한국지질자원연구원 기후변화대응연구본부 지하수환경연구센터
    2과학기술연합대학원대학교 지질과학전공
    3㈜한울생명과학
    4한국농어촌공사 농어촌연구원 미래농어촌연구소
    5서울대학교 농생명과학공동기기원

  • This article is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In this study, the TPH(Total Petroleum Hydrocarbon) contamination and microbial ecological characteristics in petroleum-contaminated site were investigated through the correlation among the vertical TPH contamination distribution of the site, the geochemical characteristics, and the indigenous microbial ecology. The high TPH concentration showed in the vicinity of 3~4 m or less which is thought to be affected by vertical movement due to the impervious clay layer. In addition, the TPH concentration was found to have a positive correlation with Fe2+, TOC concentration, and the number of petroleum-degrading bacteria, and a negative correlation with the microbial community diversity. The microbial community according to the vertical distribution of TPH showed that Proteobacteria and Firmicutes at the phylum level were dominant in this study area as a whole, and they competed with each other. In particular, it was confirmed that the difference in the microbial community was different due to the difference in the degree of vertical TPH contamination. In addition, the genera Acidovorax, Leptolinea, Rugoshibacter, and Smithella appeared dominant in the samples in which TPH was detected, which is considered to be the microorganisms involved in the degradation of TPH in this study area. It is expected that this study can be used as an important data to understand the contamination characteristics and biogeochemical and microbial characteristics of these TPH-contaminated sites.


Keywords: TPH (Total Petroleum Hydrocarbon), Ferrous iron, Microbial community, Proteobacteria, Firmicutes

This Article

  • 2022; 27(S1): 51-63

    Published on Jul 31, 2022

  • 10.7857/JSGE.2022.27.S.051
  • Received on Jun 17, 2022
  • Revised on Jun 22, 2022
  • Accepted on Jul 18, 2022

Correspondence to

  • Hee Sun Moon

  • 1Groundwater Environment Research Center, Climate Change Response Division, Korea Institute of Geoscience and Mineral Resources (KIGAM)
    2Geological Science, University of Science and Technology (UST)

  • E-mail: hmoon@kigam.re.kr