- Optimization of As Bioleaching by Herbaspirillum sp. GW103 Coupled with Coconut Oil Cake
- Govarthanan, Muthusamy;Praburaman, Loganathan;Kim, Jin-Won;Oh, Sae-Gang;Kamala-Kannan, Seralathan;Oh, Byung-Taek;
- Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University;Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University;Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University;Mine Reclamation Corp.;Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University;Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University;
Abstract
The objective of this study was to optimize the experimental conditions for bioleaching of arsenic (As) using Herbaspirillum sp. GW103 and to understand the interaction between bacteria and As during bioleaching. Five variables, temperature, time, CaCO3, coconut oil cake, and shaking rate, were optimized using response surface methodology (RSM) based Box-Behnken design (BBD). Maximum (73.2%) bioleaching of As was observed at 30℃, 60 h incubation, 1.75% CaCO3, 3% coconut oil cake, and 140 rpm. Sequential extraction of bioleached soil revealed that the isolate Herbaspirillum sp. GW103 significantly reduced 28.6% of water soluble fraction and increased 38.8% of the carbonate fraction. The results of the study indicate that the diazotrophic bacteria Herbaspirillum sp. could be used for bioleaching As from mine soil.
Keywords: Arsenic;Bioleaching;Box-Behnken design;Herbaspirillum sp.;Heavy metals fraction;
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