• Microbial Amelioration of Acid Mine Drainage Impaired Soil using the Bacterial Consortia of Klebsiella sp. and Raoultella sp.
  • Seon Yeong Park1·Gi Won Lee2·Chang Gyun Kim1,2,*

  • 1Program in Environmental and Polymer Engineering, INHA University, Incheon 22212, Korea
    2Department of Environmental Engineering, INHA University, Incheon 22212, Korea

  • This article is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


Acid mine drainage (AMD) resulting from pyrite oxidation in mining areas, subsequently leads to soil acidification accompanied by lowering pH and high concentration of metals and metalloids in its surrounding environment. Regarding to this, the microbial amelioration has been considered as a promising option for a more cost-effective and eco-friendlier countermeasure, compared to the use of alkaline chemicals. This study was aimed to evaluate influencing factors in microbially-mediated amelioration of acidic soil spiked by simulated AMD. For this, microcosm experiments were conducted by acid-neutralizing bacterial consortium (dominated by Klebsiella sp. and Raoultella sp.) under the various conditions of AMD spikes (0-2,500 mg SO42-/L), together with acidic mine soil (0-100 g) or sphagnum peat (0-5 g) in the 200 mL of nutrient medium. The employed bacterial consortium, capable of resisting to high level of sulfate concentration (up to 1,500 mg SO42-/L) in low pH, generated the ammonium while concomitantly reduced the sulfate, subsequently contributing to the effective soil stabilization with an evolution of soil pH up to neutral. Furthermore, it demonstrates that suitable condition has to be tuned for successful microbial metabolism to facilitate with neutralization during practical application.

Keywords: Acid mine drainage, Acid-neutralization, Bioremediation, Deamination, Sulfate

This Article

  • 2021; 26(1): 34-44

    Published on Feb 28, 2021

  • 10.7857/JSGE.2021.26.1.034
  • Received on Jan 6, 2021
  • Revised on Jan 9, 2021
  • Accepted on Feb 3, 2021

Correspondence to

  • Chang Gyun Kim
  • 1Program in Environmental and Polymer Engineering, INHA University, Incheon 22212, Korea

  • E-mail: cgk@inha.ac.kr