• Denitrification by a Heterotrophic Denitrifier with an Aid of Slowly Released Molasses
  • Lee, Byung-Sun;Lee, Kyu-Yeon;Shin, Do-Yun;Choi, Jong-Hak;Kim, Young-Jin;Nam, Kyoung-Phile;
  • Rural Research Institute, Korea Rural Community Corporation;Dept. of Civil and Environmental Engineering, Seoul National University;Dept. of Civil and Environmental Engineering, Seoul National University;Rural Research Institute, Korea Rural Community Corporation;Dept. of Civil and Environmental Engineering, Seoul National University;Dept. of Civil and Environmental Engineering, Seoul National University;
  • 고체 당밀정화제와 종속영양 탈질미생물을 이용한 질산염 제거
  • 이병선;이규연;신도연;최종학;김영진;남경필;
  • 한국농어촌공사 농어촌연구원;서울대학교 건설환경공학부;서울대학교 건설환경공학부;한국농어촌공사 농어촌연구원;서울대학교 건설환경공학부;서울대학교 건설환경공학부;
Abstract
This study was conducted to determine the potential applicability of slowly released molasses (SRM) to treat nitratecontaminated groundwater. SRM was made by dispersing molasses in hydroxy propyl methyl cellulose-silicamicrocrystalline cellulose matrix. Column test indicated that SRM could continuously release molasses with slowly decreasing release rates of $64.6mg-COD/L{\cdot}h$ up to 65 hrs, $12.1mg-COD/L{\cdot}h$ up to 215 hrs, and $4.4mg-COD/L{\cdot}h$up to 361 hrs. A batch test using an isolated indigenous heterotrophic denitrifier Pseudomonas sp. KY1 having nitrite reductase (nirK) and liquid molasses demonstrated that the bacterium decreased 100 mg-N/L of nitrate to less than 10 mg-N/L at the C/N ratio of 10/1 in 48 hours. In a Pseudomonas sp. KY1-attached Ottawa sand column which continuously received molasses from a SRM-containing reservoir, the bacterium successfully removed nitrate from 20 mg-N/L to 3 mg-N/L during the 361 hours of column operation. The results showed the possibility that SRM can be used as a reliable, longterm extra carbon source for indigenous heterotrophic denitrifiers.

Keywords: Slowly released molasses (SRM);Pseudomonas sp. KY1;Heterotrophic denitrification;Nitrate;

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This Article

  • 2010; 15(4): 30-38

    Published on Aug 31, 2010

  • Received on Apr 2, 2010
  • Accepted on Jul 26, 2010