• Monitoring of Geothermal Systems Wells and Surrounding Area using Molecular Biological Methods for Microbial Species
  • Ahn, Chang-Min;Han, Ji-Sun;Kim, Chang-Gyun;Park, Yu-Chul;Mok, Jong-Koo;Jang, Bum-Ju;
  • Department of Environmental Engineering, Inha University;Department of Environmental Engineering, Inha University;Department of Environmental Engineering, Inha University;Department of Geophysics, Kangwon National University;Geo3eco Co., Ltd.;Geo3eco Co., Ltd.;
  • 분자생물학적 방법을 이용한 지열시스템 관정 및 주변지역 미생물종 모니터링
  • 안창민;한지선;김창균;박유철;목종구;장범주;
  • 인하대학교 환경공학과;인하대학교 환경공학과;인하대학교 환경공학과;강원대학교 지구물리학과;(주)지오쓰리에코;(주)지오쓰리에코;
Abstract
This study was conducted to monitor microbial species dynamics within the aquifer due to long term operation of geothermal heat pump system. The species were identified by molecular biological methods of 16S rDNA. Groundwater sample was collected from both open (S region) and closed geothermal recovery system (J region) along with the control. J measured and control as well as S measured found Ralstonia pickettii as dominant species at year 2010. In contrast, Rhodoferax ferrireducens was dominantly observed for the control of S. In 2011, Sediminibacterium sp. was universely identified as the dominant species regardless of the monitoring places and type of sample, i.e., measured or control. The difference in the dynamics between the measured and the control was not critically observed, but annual variation was more strikingly found. It reveals that possible environmental changes (e.g. ORP and DO) due to the operation of geothermal heat recovery system in aquifer could be more exceedingly preceded to differentiate annual variation of microbial species rather than positional differences.

Keywords: Geothermal heat pump system;Groundwater;Microbial species;16S rDNA;

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This Article

  • 2012; 17(6): 23-32

    Published on Dec 31, 2012

  • 10.7857/JSGE.2012.17.6.023
  • Received on Mar 2, 2012
  • Revised on Nov 30, 2012
  • Accepted on Dec 7, 2012