• Biodegradation Kinetics of Diesel in a Wind-driven Bioventing System
  • Liu, Min-Hsin;Tsai, Cyuan-Fu;Chen, Bo-Yan;
  • Department of Environmental Engineering and Management, Chaoyang University of Technology;Department of Environmental Engineering and Management, Chaoyang University of Technology;Department of Environmental Engineering and Management, Chaoyang University of Technology;
Abstract
Bioremediation, which uses microbes to degrade most organic pollutants in soil and groundwater, can be used in solving environmental issues in various polluted sites. In this research, a wind-driven bioventing system is built to degrade about 20,000 mg/kg of high concentration diesel pollutants in soil-pollution mode. The wind-driven bioventing test was proceeded by the bioaugmentation method, and the indigenous microbes used were Bacillus cereus, Achromobacter xylosoxidans, and Pseudomonas putida. The phenomenon of two-stage diesel degradation of different rates was noted in the test. In order to interpret the results of the mode test, three microbes were used to degrade diesel pollutants of same high concentration in separated aerated batch-mixing vessels. The data derived thereof was input into the Haldane equation and calculated by non-linear regression analysis and trial-and-error methods to establish the kinetic parameters of these three microbes in bioventing diesel degradation. The results show that in the derivation of μm (maximum specific growth rate) in biodegradation kinetics parameters, Ks (half-saturation constant) for diesel substance affinity, and Ki (inhibition coefficient) for the adaptability of high concentration diesel degradation. The Ks is the lowest in the trend of the first stage degradation of Bacillus cereus in a high diesel concentration, whereas Ki is the highest, denoting that Bacillus cereus has the best adaptability in a high diesel concentration and is the most efficient in diesel substance affinity. All three microbes have a degradation rate of over 50% with regards to Pristane and Phytane, which are branched alkanes and the most important biological markers.

Keywords: Passive bioventing;Bacillus cereus;Achromobacter xylosoxidans;Pseudomonas putida;Haldane equation;

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This Article

  • 2016; 21(5): 8-15

    Published on Oct 31, 2016

  • 10.7857/JSGE.2016.21.5.008
  • Received on Oct 4, 2016
  • Revised on Oct 15, 2016
  • Accepted on Oct 28, 2016