Enhanced Biodegradation of Total Petroleum Hydrocarbons (TPHs) in Contaminated Soil using Biocatalyst

doi:10.7857/JSGE.2015.20.5.047

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2015 Vol.20, Issue 5 Preview Page

Enhanced Biodegradation of Total Petroleum Hydrocarbons (TPHs) in Contaminated Soil using Biocatalyst: Owen, Jeffrey S.;Pyo, Sunyeon;Kang, Guyoung;

31 October 2015. pp. 47 ~ 51

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Abstract

Biocatalytic degradation of total petroleum hydrocarbons (TPHs) in contaminated soil by hemoglobin and hydrogen peroxide is an effective soil remediation method. This study used a laboratory soil reactor experiment to evaluate the effectiveness of a nonspecific biocatalytic reaction with hemoglobin and H₂O₂ for treating TPH-contaminated soil. We also quantified changes in the soil microbial community using real-time PCR analysis during the experimental treatment. The results show that the measured rate constant for the reaction with added hemoglobin was 0.051/day, about 3.5 times higher than the constant for the reaction with only H₂O₂ (0.014/day). After four weeks of treatment, 76% of the initial soil TPH concentration was removed with hemoglobin and hydrogen peroxide treatment. The removal of initial soil TPH concentration was 26% when only hydrogen peroxide was used. The soil microbial community, based on 16S rRNA gene copy number, was higher (7.1 × 10⁶ copy number/g of bacteria, and 7.4 × 10⁵ copy number/g of Archaea, respectively) in the hemoglobin catalyzed treatment. Our results show that TPH treatment in contaminated soil using hemoglobin catalyzed oxidation led to the enhanced removal effectiveness and was non-toxic to the native soil microbial community in the initial soil.

Keywords

Hemoglobin;Hydrogen peroxide;Soil remediation;Total petroleum hydrocarbon (TPH);Real time PCR;

References

Casamayor, E., Massana, R., Benlloch, S., Øvreås, L., Díez, B., and Goddard, V.J., 2002, Changes in archaeal, bacterial and eukaryal assemblages along a salinity gradient by comparison of genetic fingerprinting methods in a multipond solar saltern. Environ. Microbiol., 4, 338-348.10.1046/j.1462-2920.2002.00297.x
Chen, S., Stevens, D.K., and Kang, G., 1999, Pentachlorophenol and crystal violet degradation in water and soils using heme and hydrogen peroxide, Wat. Res., 33(17), 3657-3665.10.1016/S0043-1354(99)00262-6
Chen, S., Stevens, D.K., Kang, G., and Hsieh, M., 2006, Treating soil PCP at optimal conditions using heme and peroxide, J. Environ. Eng., ASCE, 132(7), 704-708.10.1061/(ASCE)0733-9372(2006)132:7(704)
Chen, S., Stevens, D.K., Kang, G., Hsu, J., and Wu, S., 2009, Kinetics of pentachlorophenol degradation in soil using heme and peroxide, J. Environ. Eng., ASCE, 135(4), 279-284.10.1061/(ASCE)0733-9372(2009)135:4(279)
Chung, N., Park, K., Stevens, S.K., and Kang, G., 2014, Verification of heme catalytic cycle with 5-aminosalicylic acid and its application to soil remediation of polycyclic aromatic hydrocarbons, Environ. Eng. Res., 19(2), 139-143.10.4491/eer.2014.19.2.139
Danner, D.J., Brognac, P.J., Arceneaux, D., and Chandra Patel, V., 1973, The oxidation of phenol and its reaction product by horseradish peroxidase and hydrogen peroxide, Arch. Biochem. Biophys., 156, 759-763.10.1016/0003-9861(73)90329-9
Hong, J. and Cho, J., 2015, Environmental variables shaping the ecological niche of Thaumarchaeota in soil: Direct and indirect causal effects, PLoS One, 10(8):e0133763. doi:10.1371, 1-20.10.1371/journal.pone.0133763
Kang, G., Park, K., Cho, J., Stevens, D.K., and Chung, N., 2015, Remediation of polycyclic aromatic hydrocarbons in soil using hemoglobin-catalytic mechanism, J. Environ. Eng., ASCE, ISSN 0733-9372/04015025-1-5. doi:10.1061/(ASCE)EE.1943-7870.0000955.10.1061/(ASCE)EE.1943-7870.0000955
Ministry of Environment (Korea), 2009, Official Test Method on Soil Pollution, ES 07552.1, 174-185.
Nadkarni, M.A., Martin, F.E., Jacques, N.a., and Hunter, N., 2002, Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set, Microbiology, 148, 257-266.10.1099/00221287-148-1-257
Oh, S.Y. and Shin, D.S., 2013, Treatment of diesel-contaminated soil by Fenton and persulfate oxidation with zero-valent iron, Soil Sed. Contam., 23(2), 180-193.
Palmroth, M., Langwaldt, J.H., Aunola, T.A., Goi, A., Puhakka, J.A., and Tuhkanen, T.A., 2006, Treatment of PAH-contaminated soil by combination of Fenton’s reaction and biodegradation, J. Chem. Technol. Biotechnol., 81, 598-607.10.1002/jctb.1476
Rice, R.H., Lee, Y.M., and Brown, W.D., 1983, Interactions of heme protein with hydrogen peroxide: Protein cross-linking and covalent binding of benzo[a]pyrene and 17B-estradiol, Arch. Biochem. Biophys., 221(2), 417-427.10.1016/0003-9861(83)90160-1
Stahl, D.A. and Amann, R.I., 1991, Development and application of nucleic acid probes. In: E. Stackebrandt and M. Goodfellow (eds.), Nucleic Acid Techniques in Bacterial Systematics, John Wiley & Sons, New York, p. 205-248.
Takai, K. and Horikoshi, K., 2000, Rapid detection and quantification of members of the archaeal community by quantitative PCR using fluorogenic probes, Appl. Environ. Microbiol., 66, 5066-5072.10.1128/AEM.66.11.5066-5072.2000
Umezawa, T. and Higuchi, T., 1989, Aromatic ring cleavage by lignin peroxidase. In: N.G. Lewis and M.G. Paice (eds.), Plant Cell Wall Polymers Biogenesis and Biodegradation, American Chemical Society, Washington, DC, p. 503-518.

Information

Publisher :The Korean Society of Soil and Groundwater Environment
Publisher(Ko) :한국지하수토양환경학회
Journal Title :Journal of Soil and Groundwater Environment
Journal Title(Ko) :지하수토양환경
Volume : 20
No :5
Pages :47 ~ 51
DOI :https://doi.org/10.7857/JSGE.2015.20.5.047

[1] Casamayor, E., Massana, R., Benlloch, S., Øvreås, L., Díez, B., and Goddard, V.J., 2002, Changes in archaeal, bacterial and eukaryal assemblages along a salinity gradient by comparison of genetic fingerprinting methods in a multipond solar saltern. Environ. Microbiol., 4, 338-348.10.1046/j.1462-2920.2002.00297.x

[2] Chen, S., Stevens, D.K., and Kang, G., 1999, Pentachlorophenol and crystal violet degradation in water and soils using heme and hydrogen peroxide, Wat. Res., 33(17), 3657-3665.10.1016/S0043-1354(99)00262-6

[3] Chen, S., Stevens, D.K., Kang, G., and Hsieh, M., 2006, Treating soil PCP at optimal conditions using heme and peroxide, J. Environ. Eng., ASCE, 132(7), 704-708.10.1061/(ASCE)0733-9372(2006)132:7(704)

[4] Chen, S., Stevens, D.K., Kang, G., Hsu, J., and Wu, S., 2009, Kinetics of pentachlorophenol degradation in soil using heme and peroxide, J. Environ. Eng., ASCE, 135(4), 279-284.10.1061/(ASCE)0733-9372(2009)135:4(279)

[5] Chung, N., Park, K., Stevens, S.K., and Kang, G., 2014, Verification of heme catalytic cycle with 5-aminosalicylic acid and its application to soil remediation of polycyclic aromatic hydrocarbons, Environ. Eng. Res., 19(2), 139-143.10.4491/eer.2014.19.2.139

[6] Danner, D.J., Brognac, P.J., Arceneaux, D., and Chandra Patel, V., 1973, The oxidation of phenol and its reaction product by horseradish peroxidase and hydrogen peroxide, Arch. Biochem. Biophys., 156, 759-763.10.1016/0003-9861(73)90329-9

[7] Hong, J. and Cho, J., 2015, Environmental variables shaping the ecological niche of Thaumarchaeota in soil: Direct and indirect causal effects, PLoS One, 10(8):e0133763. doi:10.1371, 1-20.10.1371/journal.pone.0133763

[8] Kang, G., Park, K., Cho, J., Stevens, D.K., and Chung, N., 2015, Remediation of polycyclic aromatic hydrocarbons in soil using hemoglobin-catalytic mechanism, J. Environ. Eng., ASCE, ISSN 0733-9372/04015025-1-5. doi:10.1061/(ASCE)EE.1943-7870.0000955.10.1061/(ASCE)EE.1943-7870.0000955

[9] Ministry of Environment (Korea), 2009, Official Test Method on Soil Pollution, ES 07552.1, 174-185.

[10] Nadkarni, M.A., Martin, F.E., Jacques, N.a., and Hunter, N., 2002, Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set, Microbiology, 148, 257-266.10.1099/00221287-148-1-257

[11] Oh, S.Y. and Shin, D.S., 2013, Treatment of diesel-contaminated soil by Fenton and persulfate oxidation with zero-valent iron, Soil Sed. Contam., 23(2), 180-193.

[12] Palmroth, M., Langwaldt, J.H., Aunola, T.A., Goi, A., Puhakka, J.A., and Tuhkanen, T.A., 2006, Treatment of PAH-contaminated soil by combination of Fenton’s reaction and biodegradation, J. Chem. Technol. Biotechnol., 81, 598-607.10.1002/jctb.1476

[13] Rice, R.H., Lee, Y.M., and Brown, W.D., 1983, Interactions of heme protein with hydrogen peroxide: Protein cross-linking and covalent binding of benzo[a]pyrene and 17B-estradiol, Arch. Biochem. Biophys., 221(2), 417-427.10.1016/0003-9861(83)90160-1

[14] Stahl, D.A. and Amann, R.I., 1991, Development and application of nucleic acid probes. In: E. Stackebrandt and M. Goodfellow (eds.), Nucleic Acid Techniques in Bacterial Systematics, John Wiley & Sons, New York, p. 205-248.

[15] Takai, K. and Horikoshi, K., 2000, Rapid detection and quantification of members of the archaeal community by quantitative PCR using fluorogenic probes, Appl. Environ. Microbiol., 66, 5066-5072.10.1128/AEM.66.11.5066-5072.2000

[16] Umezawa, T. and Higuchi, T., 1989, Aromatic ring cleavage by lignin peroxidase. In: N.G. Lewis and M.G. Paice (eds.), Plant Cell Wall Polymers Biogenesis and Biodegradation, American Chemical Society, Washington, DC, p. 503-518.

Journal of Soil and Groundwater Environment ISSN:1598-6438(Print) 2287-8831(Online) 지하수토양환경

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