All Issue

2015 Vol.20, Issue 7 Preview Page
Comparison in Structural Characteristics and Phenanthrene Sorption of Molecular Size-Fractionated Humic Acids

분자량 크기별 토양 휴믹산(HA)의 구조적 특성 및 페난트렌 흡착 반응특성 비교

이두희;김소희;신현상;

Lee, Doo-Hee;Kim, So-Hui;Shin, Hyun-Sang;

31 December 2015. pp. 70 ~ 79
PDF Citation
Abstract
A sample of soil humic acid (HA) was divided by ultrafiltration (UF) into five fractions of different molecular size (UF1: > 300, UF2: 100~300, UF3: 30~100, UF4: 10~30, UF5: 1~10 kilodaltons). Apparent average molecular weight (Mw) of the HA fractions were measured using high performance size exclusion chromatography (HPSEC), and the chemical and structural properties of the five HA fractions were characterized by elemental compositions (H/C, O/C and w ((2O + 3NH)/ C)) and ultraviolet-visible absorption ratios (SUVA, A4/6). The organic carbon normalized-sorption coefficients (Koc) for the binding of phenanthrene to the HA fractions were determined by fluorescence quenching and relationship between the sorption coefficients and structural characteristics of the HA fractions were investigated. The elemental analysis and UV-vis spectral data indicated that the HA fractions with higher molecular weights have grater aliphatic character and lower contents of oxygen, while the HA fractions with lower molecular size have greater aromatic character and molecular polarity that correspond to greater SUVA and internal oxidation values (w). The log Koc values (L/kg C) were gradual increased from 4.45 for UF5 to 4.87 for UF1. The correlation study between the structural descriptors of the HA fractions and log Koc values of phenanthrene show that the magnitude of Koc values positively correlated with $M_w$ and H/C, while negatively correlated with the independent descriptors of the O/C, w, SUVA and A4/6.
Keywords
Soil humic acid;Molecular size-fractionation;Chemical-structural properties;Phenanthrene;Koc;

References
  1. Aiken, G.R., Mcknight, D.M., Wershaw, R.L., and MacCharthy, P., 1985, An Introduction to Humic Substances in Soil, Sediment, and Water, In Humic Substances in Soil, Sediment and Water: Geochemistry, Isolation, and Characterization (G. R. Aiken, D. M., MaKnight and R. L. Wershaw, eds.), John Wiley and Sons, USA.
  2. Cha, H.J., Park, D., Park, H., Kang, M.J., Lee, W., Choi, G.S., Cho, Y.H., Chung, K.H., Lee, H.P., Shin, H.S., and Lee, C.W., 2004, Vertical distribution of 137Cs and 90Sr activities in the soils of Korea, J. Korea Asso. Radiat. Prot., 29(3), 197-204.
  3. Chefetz, B. and Xing, B., 2009, Relative role of aliphatic and aromatic moieties as sorption domain for organic compounds: A review, Environ. Sci. Technol., 43, 1680-168810.1021/es803149u
  4. Chen, W.B., Johson, B.J., Chefetz, B., Zhu, L., and Xing, B., 2005, Sorption of polar and nonpolar aromatic organic contaminants by plant cuticular materials: Role of polarity and accessibility, Environ. Sci. Technol., 39(16), 6138-6146.10.1021/es050622q
  5. Chen, W.B., Smith D.S., and Gueguen, D., 2013, Infleunce of water cheistry and dissolved organic matter (DOM) molecular size on copper and mercury binding determined by multiresponse fluorescence quenching, Chemosphere, 92(4), 351-359.10.1016/j.chemosphere.2012.12.075
  6. Chin, Y., Aiken, G., and O’Loughlin, E., 1994, Molecular weight, polydispersity, and spectroscopic properties of aquatic humic substances, Environ. Sci. Technol., 28, 1853-1858.10.1021/es00060a015
  7. Chiou, C.T., 2002, Partition and adsorption of organic contaminants in environmental systems, Jhon Wiley & Sons, Hoboken, NJ
  8. Christ, L., Knicker, H., Kogel-Knabner, I., and Kretzschmar, R., 2000, Chemical heterogeneity of humic substances: characterization of size fractions obtained by hollow-fiber ultrafiltration, European J. Soil Sci., 51, 617-625.10.1111/j.1365-2389.2000.00352.x
  9. Chung, K.H., Shin, H.S., Lee, W., Cho, Y.H., Choi, G.S., and Lee, C.W., 2004, Molecular size fractionation of soil fulvic acid by gel filtration chromatography and analysis of their fluorescence characteristics, Anal. Sci. Technol., 17(2), 163-172.
  10. Cieslewicz, I. and Gonet, S.S., 2004, Properties of humic acids as biomakers of lake sediment management, Aquatic Science, 66, 178-184.10.1007/s00027-004-0702-0
  11. Conte, P. and Piccolo, A., 1999, Conformational arrangement of dissolved humic substances. Influence of solution composition on association of humic molecules, Environ. Sci. Technol. 33, 1682-1690.10.1021/es9808604
  12. Edwards, N., 1983, Polynuclear aromatic hydrocarbons (PAHs) in the terrestrial - A review, J. Environ. Qual., 12, 427-441.
  13. Hur, J. and Schlautman, M., 2004, Influence of humic substance adsorptive fractionation on pyrene partitioning to dissolved and mineral-Associated humic Substances, Environ. Sci. Technol. 38, 5871-5877.10.1021/es049790t
  14. IHSS, 2015, International Humic Substance Society Home Page, http://www.ihss.gate.edu/.
  15. Kang, S. and Xing, B., 2005, Phenanthrene sorption to sequentially extracted soil humic acids and humins, Environ. Sci. Technol., 39, 134-140.10.1021/es0490828
  16. Khalaf, M., Kohl, S.D., Klumpp, E., Rice, J.A., and Tombcz, E., 2003, Comparison of sorption domains in molecular weight fractions of a soil Humic acid using solid-state 19F NMR., Environ. Sci. Technol., 37, 2855-2860.10.1021/es0206386
  17. Laor, Y. and Rebhun, M., 1997, Complexation-flocculation: A new method to determine binding coefficients of organic contaminants to dissolved humic substances, Environ. Sci. Technol., 31, 3558-3564.10.1021/es970311i
  18. Lee, B.M., Seo, Y.S. and Hur, J., 2015, Investigation of adsorptive fractionation of humic acid on graphene oxide using fluorescence EEM-PARAFAC, Wat. Res., 73, 242-251.10.1016/j.watres.2015.01.020
  19. Lee, C.H., Shin, H.S., Chung, K.H., Cho, Y.H. and Lee, C.W., 2003, Isolation and Characterization of Humic Acids Present in Soils at the Vicinity of Domestic Atomic Power Plants (NPPs), J. Korean Asso. Radiat. Prot., 28(3), 165-172.
  20. Lee, C.H., Shin, H.S., and Kang, K.H., 2004, Chemical and spectroscopic characterization of peat moss and its different humic fractions (Humin, Humic Acid and Fulvic Acid), J. Soil Groundwater Environ., 9(4), 42-51.
  21. Lee, D.H., Eom, W.S., and Shin, H.S., 2013, A study of the correlations between molecular structures of soil humins and sorption properties of phenanthrene, J. Kor. SOc. Environ. Eng., 35(12), 897-905
  22. Lee, D.H., Lee, S.S., and Shin, H.S., 2008, Chemical and spectroscopic chanracterization of soil humic and fulvic acids and sorption coefficient of phenanthrene: A correlation study, J. Kor. SOc. Environ. Eng., 30, 1067-1074.
  23. Leenheer, J., Mcknight, D., Thurman, E., and MacCarthy, P., 1989, Humic Substances in the Suwannee River, Georgia: Interactions, Properties, and Proposed Structure, US Geology Survey, Open-File Report 85-557, Denver, Colorado.
  24. Li, L., Zhao, Z., Huang, W., Peng, P., Sheng, G., and Fu, J., 2004, Characterization of humic acids fractionated by ultrafiltration, Org. Geochem., 35, 1205-1037.
  25. Pan, B., Xing, B.S., Liu, W.X., Xing, G.H., and Tao, S., 2007, Investigating interactions of phenanthrene with dissolved organic matter: limitations of Stern-Volmer plot, Chemosphere 69, 1555-1562.10.1016/j.chemosphere.2007.05.059
  26. Peuravuori, J., 2001, Partition coefficients of pyrene to lake aquatic humic matter determined by fluorescence quenching and solubility enhancement, Analytica Chimica Acta, 429, 65-73.10.1016/S0003-2670(00)01258-7
  27. Rao, L. and Choppin, G.R., 1994, Effect of natural organic materials on cadium and neptunium, Radiochim Acta., 69, 87-95.
  28. Salloum, M.J., Chefetz, B., and Hatcher, P.G., 2002, Phenanthrene sorption by aliphatic-rich organic matter, Environ. Sci. Technol, 36, 1953-1958.10.1021/es015796w
  29. Shin, H.S., Lee, C.H., Chung, K.H., and Lee, C.W, 2003, Characterization of humic and fulvic acid extracted fro soils in different depth: Proton exchange capacity, elemental composition and 13C NMR spectrum, Anal. Sci. Tech,, 16(4), 283-291.
  30. Shin, H.S., Monsallier, J., and Choppin, G., 1999, Spectroscopic and chemical characterizations of molecular size fractionated humic acid, Talanta, 50, 641-647.10.1016/S0039-9140(99)00161-7
  31. Sun, K., Jin, J., Kang, M., Zhang, Z., Pan, Z., Wang, Z., Wu, F., and Xing, B., 2013, Isolation and characterization of different organic matter fractions from a same soil source and their phenanthrene sorption, Environ, Sci. Technol., 47, 5138-5145.10.1021/es3052087
  32. Thurman, E.M., Wershaw, R.L., Malcolm, P.L., and Pinckney, D.J., 1982, Molecular size of aquatic humic substances, Org. Geochem., 4, 27-35.10.1016/0146-6380(82)90005-5
  33. Trubetskaya, O., Trubetskoj, O., and Richard, C., 2014, Hydrophobicity of electrophretic fractions of different soil humic acids, J. Soil Sed., 14, 292-297.10.1007/s11368-013-0667-5
  34. Weishaar, J., Aiken, G., Bergamaschi, B., Fram, M., Fujii, R., and Mopper, K., 2003, Evaluation of specific ultraviolet absorbance as an indicator of the chemical composition and reactivity of dissolved organic carbon, Environ. Sci. Technol., 37, 4702- 4708.10.1021/es030360x
  35. Zhou, Q., Cabaniss, S., and Maurice, P., 2000, Considerations in the use of high-pressure size exclusion chromatography (HPSEC) for determining molecular weights of aquatic humic substances, Water Research, 34, 3505-3514.10.1016/S0043-1354(00)00115-9
Information
  • Publisher :The Korean Society of Soil and Groundwater Environment
  • Publisher(Ko) :한국지하수토양환경학회
  • Journal Title :Journal of Soil and Groundwater Environment
  • Journal Title(Ko) :지하수토양환경
  • Volume : 20
  • No :7
  • Pages :70 ~ 79
  • DOI :https://doi.org/10.7857/JSGE.2015.20.7.070
Journal Informaiton Journal of Soil and Groundwater Environment Journal of Soil and Groundwater Environment
Online Submission
  • KOFST
  • KISTI Current Status
  • KISTI Cited-by
  • orcid
  • open access
  • ccl
Journal Informaiton Journal Informaiton - close