Analysis of Soil Properties and Microbial Communities for Mine Soil Vegetation

doi:10.7857/JSGE.2015.20.3.083

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2015 Vol.20, Issue 3 Preview Page

Analysis of Soil Properties and Microbial Communities for Mine Soil Vegetation 폐광산지역 토양 식생복원 과정 내 토양특성 및 미생물 군집 변화 분석: 박민정;윤민호;남인현;
Park, Min-Jeong;Yoon, Min-Ho;Nam, In-Hyun;

30 June 2015. pp. 83 ~ 91

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Abstract

Mine soil contamination by high levels of metal ions that prevents the successful vegetation poses a serious problem. In the study presented here, we used the microbial biocatalyst of urease producing bacterium Sporosarcina pasteurii or plant extract based BioNeutro-GEM (BNG) agent. The ability of the biocatalysts to bioremediate contaminated soil from abandoned mine was examined by solid-state composting vegetation under field conditions. Treatment of mine soil with the 2 biocatalysts for 5 months resulted in pH increase and electric conductivity reduction compared to untreated control. Further analyses revealed that the microbial catalysts also promoted the root and shoot growth to the untreated control during the vegetation treatments. After the Sporosarcina pasteurii or plant extract based BNG treatment, the microbial community change was monitored by culture-independent pyrosequencing. These results demonstrate that the microbial biocatalysts could potentially be used in the soil bioremediation from mine-impacted area.

Keywords

Mine soil vegetation;Urease

Plant extract;$CaCO_3$ precipitation;Microbial community analysis;

References

Bamhisel, R. and Bertsch, P.M., 1982, Method of Soil Analysis Part2 Chemical and Microbiological Properties, 2nd edition, Dinauer, R.C., Gates, K.E., Buxton, D.R., Madison, Wisconsin, USA, 282 p.
Bang, S.S., Galinat, J.K., and Ramakrishnan, V., 2001, Calcite precipitation induced by polyurethane immobilized Bacillus pasteurii, Enzyme Microb. Technol., 28, 404-409.10.1016/S0141-0229(00)00348-3
Choi, G.S., Kim, T.H., and Kwon, H.H., 2009, A study on the protection method of mine site slope using mine green framework, J. Kor. Soc. Rock Mechanics, 19, 526-533.
Conrad, R., 1996, Soil microorganisms as controllers of atmospheric trace gases (H₂, CO, CH₄, OCS, N₂O, and NO), Microbiol. Rev. 60, 609-640.
DeJong, J.T., Fritzges, M.B., and Nusslein, K., 2006, Microbially induced cementation to control sand response to undrained shear, J. Geotech. Geoenviron., 132, 1381-1392.10.1061/(ASCE)1090-0241(2006)132:11(1381)
De Muynck, W., De belie, N., and Verstraete, W., 2010, Microbial carbonate precipitation in construction materials: A review, Ecol. Eng. 36, 118-136.10.1016/j.ecoleng.2009.02.006
Gobet, A., Böer, S.I., Huse, S.M., van Beusekom, J.E., Quince, C., Sogin, M.L., Boetius, A., and Ramette, A., 2012, Diversity and dynamics of rare and of resident bacterial populations in coastal sands, ISME J., 6, 542-553.10.1038/ismej.2011.132
Hong, S.H., Lee, Y.H., Na, C.S., Kim, D.Y., Kim, J.G., Kang, B.H., and Shim, S.I., 2010, Analysis of weed vegetation in vicinity of abandoned mines, Kor. J. Weed Sci., 30, 17-24.10.5660/KJWS.2010.30.1.017
Hur, M.S., Kim, Y.H., Song, H.R., Kim, J.M., Choi, Y.I., and Yi, H.N., 2011, Effect of Genetically Modified Poplars on Soil Microbial Communities during the Phytoremediation of Waste Mine Tailings, J. Appl. Environ. Microbiol., 77, 7611-7619.10.1128/AEM.06102-11
Jung, M.H., Ryu, J.H., Kim, T.H., Lee, H.J., Choo, C.O., Shin, Y.S., and Jeong, G.C., 2012, Development of green rehabilitation material for rock and abandoned mine debris slopes, J. Eng. Geol., 22, 439-448.10.9720/kseg.2012.4.439
Kang, C.H., Oh, S.J., Shin, Y., Han, S.H., Nam, I.H., and So, J.S., 2015, Bioremediation of lead by ureolytic bacteria isolated from soil at abandoned mines in South Korea, Ecol. Eng., 74, 402-407.10.1016/j.ecoleng.2014.10.009
Kennedy, A. C. and Smith, K.L., 1995, Soil microbial diversity and the sustainability of agricultural soils, Plant Soil, 170, 75-86.10.1007/BF02183056
Lee, M.N. and Park, H.D., 2012, Isolation and characterization of acidophilic yeasts producing urease from Korean traditional Nuruk, Korean J. Food Preserv., 19, 308-314.10.11002/kjfp.2012.19.2.308
Leloup, J., Quillet, L., Berthe, T., and Petit, F., 2006, Diversity of the dsrAB (dissimilatory sulfitereductase) gene sequences retrieved from two contrasting mudflats of the Seine estuary, FEMS Microbiol.Ecol., 55, 230-238.10.1111/j.1574-6941.2005.00021.x
Le Metayer-Levrel, G., Castanier, S., Orial, G., Loubiere, J.F., and Perthuisot, J.P., 1999, Applications of bacterial carbonatogenesis to the protection and regeneration of limestones in buildings and historic patrimony, Sediment Geol., 126, 25-34.10.1016/S0037-0738(99)00029-9
Li, L., Qian, C., Cheng, L., and Wang, R., 2010, A laboratory investigation of microbe-inducing CdCO₃ precipitate treatment in Cd²⁺ contaminated soil, J. Soil Sediment, 10, 248-254.10.1007/s11368-009-0089-6
McLellan, S.L., Huse, S.M., Mueller-Spitz, S.R., Andreishcheva, E.N., and Sogin, M.L., 2010, Diversity and population structure of sewage-derived microorganisms in wastewater treatment plant influent, Environ. Microbiol., 12, 378-392.10.1111/j.1462-2920.2009.02075.x
Min, J.G., Park, E.H., Moon, H.S., and Kim, J.K., 2005, Chemical properties and heavy metal content of forest soils around abandoned coal mine lands in the Mungyeong area, Korean J. Agric. For. Meteorol., 7, 265-273.
Mobley, H.L. and Hausinger, R.P., 1989, Microbial ureases: Significance, regulation, and molecular characterization, Microbiol. Rev., 53, 85-108.
Park, S.S., Kim, W.J., and Lee, J.C., 2011, Effect of biomineralization on the strength of cemented sands, J. Korean Geotech. Soc., 27, 75-84.
Roh, S.B., Park, M.J., Chon, C.M., Kim, J.G., Song, H., Yoon, M.H., and Nam, I.H., 2015, Bioremediation of heavy metal contaminated mine wastes using urease based plant extract, J. Soil Groundw. Environ., 20, 56-64.10.7857/JSGE.2015.20.1.056
Riddles, P.W., Whan, V., Blakeley, R.L., and Zerner, B., 1991, Cloning and sequencing of a jack bean urease-encoding cDNA, Gene, 108, 265-267.10.1016/0378-1119(91)90443-F
Ward, N.L, Challacombe, J.F., Janssen, P.H., Henrissat, B., Coutinho, P.M., Wu, M., Xie, G., Haft, D.H., Sait, M., Badger, J., Barabote, R.D., Bradley, B., Brettin, T.S., Brinkac, L.M., Bruce D., Creasy T., Daugherty, S.C., Davidsen, T.M, Deboy, R.T., Detter, J.C., Dodson, R.J., Durkin A.S., Granpathy, A., Gwinn-Giglio, M., Han, C.S., Khouri, H., Kiss, H., Kothari, S.P., Madupu, R., Nelson, K.E., Nelson, W.C., Paulsen, I., Penn, K., Ren, Q., Rosovitz, M.J., Selengut, J.D., Shrivastava, S., Sullivan S.A., Tapia, R., Thompson, L.S., Watkins, K.L., Yang, Q., Yu, C., Zafar, N., Zhou, L., and Kuske, C.R., 2009, Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils, Appl. Environ. Microbiol., 75, 2046-2056.10.1128/AEM.02294-08

Information

Publisher :The Korean Society of Soil and Groundwater Environment
Publisher(Ko) :한국지하수토양환경학회
Journal Title :Journal of Soil and Groundwater Environment
Journal Title(Ko) :지하수토양환경
Volume : 20
No :3
Pages :83 ~ 91
DOI :https://doi.org/10.7857/JSGE.2015.20.3.083

[1] Bamhisel, R. and Bertsch, P.M., 1982, Method of Soil Analysis Part2 Chemical and Microbiological Properties, 2nd edition, Dinauer, R.C., Gates, K.E., Buxton, D.R., Madison, Wisconsin, USA, 282 p.

[2] Bang, S.S., Galinat, J.K., and Ramakrishnan, V., 2001, Calcite precipitation induced by polyurethane immobilized Bacillus pasteurii, Enzyme Microb. Technol., 28, 404-409.10.1016/S0141-0229(00)00348-3

[3] Choi, G.S., Kim, T.H., and Kwon, H.H., 2009, A study on the protection method of mine site slope using mine green framework, J. Kor. Soc. Rock Mechanics, 19, 526-533.

[4] Conrad, R., 1996, Soil microorganisms as controllers of atmospheric trace gases (H₂, CO, CH₄, OCS, N₂O, and NO), Microbiol. Rev. 60, 609-640.

[5] DeJong, J.T., Fritzges, M.B., and Nusslein, K., 2006, Microbially induced cementation to control sand response to undrained shear, J. Geotech. Geoenviron., 132, 1381-1392.10.1061/(ASCE)1090-0241(2006)132:11(1381)

[6] De Muynck, W., De belie, N., and Verstraete, W., 2010, Microbial carbonate precipitation in construction materials: A review, Ecol. Eng. 36, 118-136.10.1016/j.ecoleng.2009.02.006

[7] Gobet, A., Böer, S.I., Huse, S.M., van Beusekom, J.E., Quince, C., Sogin, M.L., Boetius, A., and Ramette, A., 2012, Diversity and dynamics of rare and of resident bacterial populations in coastal sands, ISME J., 6, 542-553.10.1038/ismej.2011.132

[8] Hong, S.H., Lee, Y.H., Na, C.S., Kim, D.Y., Kim, J.G., Kang, B.H., and Shim, S.I., 2010, Analysis of weed vegetation in vicinity of abandoned mines, Kor. J. Weed Sci., 30, 17-24.10.5660/KJWS.2010.30.1.017

[9] Hur, M.S., Kim, Y.H., Song, H.R., Kim, J.M., Choi, Y.I., and Yi, H.N., 2011, Effect of Genetically Modified Poplars on Soil Microbial Communities during the Phytoremediation of Waste Mine Tailings, J. Appl. Environ. Microbiol., 77, 7611-7619.10.1128/AEM.06102-11

[10] Jung, M.H., Ryu, J.H., Kim, T.H., Lee, H.J., Choo, C.O., Shin, Y.S., and Jeong, G.C., 2012, Development of green rehabilitation material for rock and abandoned mine debris slopes, J. Eng. Geol., 22, 439-448.10.9720/kseg.2012.4.439

[11] Kang, C.H., Oh, S.J., Shin, Y., Han, S.H., Nam, I.H., and So, J.S., 2015, Bioremediation of lead by ureolytic bacteria isolated from soil at abandoned mines in South Korea, Ecol. Eng., 74, 402-407.10.1016/j.ecoleng.2014.10.009

[12] Kennedy, A. C. and Smith, K.L., 1995, Soil microbial diversity and the sustainability of agricultural soils, Plant Soil, 170, 75-86.10.1007/BF02183056

[13] Lee, M.N. and Park, H.D., 2012, Isolation and characterization of acidophilic yeasts producing urease from Korean traditional Nuruk, Korean J. Food Preserv., 19, 308-314.10.11002/kjfp.2012.19.2.308

[14] Leloup, J., Quillet, L., Berthe, T., and Petit, F., 2006, Diversity of the dsrAB (dissimilatory sulfitereductase) gene sequences retrieved from two contrasting mudflats of the Seine estuary, FEMS Microbiol.Ecol., 55, 230-238.10.1111/j.1574-6941.2005.00021.x

[15] Le Metayer-Levrel, G., Castanier, S., Orial, G., Loubiere, J.F., and Perthuisot, J.P., 1999, Applications of bacterial carbonatogenesis to the protection and regeneration of limestones in buildings and historic patrimony, Sediment Geol., 126, 25-34.10.1016/S0037-0738(99)00029-9

[16] Li, L., Qian, C., Cheng, L., and Wang, R., 2010, A laboratory investigation of microbe-inducing CdCO₃ precipitate treatment in Cd²⁺ contaminated soil, J. Soil Sediment, 10, 248-254.10.1007/s11368-009-0089-6

[17] McLellan, S.L., Huse, S.M., Mueller-Spitz, S.R., Andreishcheva, E.N., and Sogin, M.L., 2010, Diversity and population structure of sewage-derived microorganisms in wastewater treatment plant influent, Environ. Microbiol., 12, 378-392.10.1111/j.1462-2920.2009.02075.x

[18] Min, J.G., Park, E.H., Moon, H.S., and Kim, J.K., 2005, Chemical properties and heavy metal content of forest soils around abandoned coal mine lands in the Mungyeong area, Korean J. Agric. For. Meteorol., 7, 265-273.

[19] Mobley, H.L. and Hausinger, R.P., 1989, Microbial ureases: Significance, regulation, and molecular characterization, Microbiol. Rev., 53, 85-108.

[20] Park, S.S., Kim, W.J., and Lee, J.C., 2011, Effect of biomineralization on the strength of cemented sands, J. Korean Geotech. Soc., 27, 75-84.

[21] Roh, S.B., Park, M.J., Chon, C.M., Kim, J.G., Song, H., Yoon, M.H., and Nam, I.H., 2015, Bioremediation of heavy metal contaminated mine wastes using urease based plant extract, J. Soil Groundw. Environ., 20, 56-64.10.7857/JSGE.2015.20.1.056

[22] Riddles, P.W., Whan, V., Blakeley, R.L., and Zerner, B., 1991, Cloning and sequencing of a jack bean urease-encoding cDNA, Gene, 108, 265-267.10.1016/0378-1119(91)90443-F

[23] Ward, N.L, Challacombe, J.F., Janssen, P.H., Henrissat, B., Coutinho, P.M., Wu, M., Xie, G., Haft, D.H., Sait, M., Badger, J., Barabote, R.D., Bradley, B., Brettin, T.S., Brinkac, L.M., Bruce D., Creasy T., Daugherty, S.C., Davidsen, T.M, Deboy, R.T., Detter, J.C., Dodson, R.J., Durkin A.S., Granpathy, A., Gwinn-Giglio, M., Han, C.S., Khouri, H., Kiss, H., Kothari, S.P., Madupu, R., Nelson, K.E., Nelson, W.C., Paulsen, I., Penn, K., Ren, Q., Rosovitz, M.J., Selengut, J.D., Shrivastava, S., Sullivan S.A., Tapia, R., Thompson, L.S., Watkins, K.L., Yang, Q., Yu, C., Zafar, N., Zhou, L., and Kuske, C.R., 2009, Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils, Appl. Environ. Microbiol., 75, 2046-2056.10.1128/AEM.02294-08

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