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2021 Vol.26, Issue 1 Preview Page
Microbial Amelioration of Acid Mine Drainage Impaired Soil using the Bacterial Consortia of Klebsiella sp. and Raoultella sp.
Seon Yeong Park1
,
Gi Won Lee2
,
Chang Gyun Kim12*
1Program in Environmental and Polymer Engineering, INHA University, Incheon 22212, Korea
2Department of Environmental Engineering, INHA University, Incheon 22212, Korea
*Corresponding Author
28 February 2021. pp. 34-44
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Abstract

Acid mine drainage (AMD) resulting from pyrite oxidation in mining areas, subsequently leads to soil acidification accompanied by lowering pH and high concentration of metals and metalloids in its surrounding environment. Regarding to this, the microbial amelioration has been considered as a promising option for a more cost-effective and eco-friendlier countermeasure, compared to the use of alkaline chemicals. This study was aimed to evaluate influencing factors in microbially-mediated amelioration of acidic soil spiked by simulated AMD. For this, microcosm experiments were conducted by acid-neutralizing bacterial consortium (dominated by Klebsiella sp. and Raoultella sp.) under the various conditions of AMD spikes (0-2,500 mg SO42-/L), together with acidic mine soil (0-100 g) or sphagnum peat (0-5 g) in the 200 mL of nutrient medium. The employed bacterial consortium, capable of resisting to high level of sulfate concentration (up to 1,500 mg SO42-/L) in low pH, generated the ammonium while concomitantly reduced the sulfate, subsequently contributing to the effective soil stabilization with an evolution of soil pH up to neutral. Furthermore, it demonstrates that suitable condition has to be tuned for successful microbial metabolism to facilitate with neutralization during practical application.

Keywords
Acid mine drainage
Acid-neutralization
Bioremediation
Deamination
Sulfate
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Information
  • Publisher :The Korean Society of Soil and Groundwater Environment
  • Publisher(Ko) :한국지하수토양환경학회
  • Journal Title :Journal of Soil and Groundwater Environment
  • Journal Title(Ko) :지하수토양환경
  • Volume : 26
  • No :1
  • Pages :34-44
  • Received Date : 2021-01-06
  • Revised Date : 2021-01-19
  • Accepted Date : 2021-02-03
  • DOI :https://doi.org/10.7857/JSGE.2021.26.1.034
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